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Details




NbTI01B04

Phenotype Description
Severely stunted plants, crinckled new growth, no apical meristem, severe cell death on top leaves,
Annotation
Solyc08g081910.2.1;genomic_reference:SL2.50ch08 gene_region:61988688-61993931 transcript_region:SL2.50ch08:61988688..61993931- functional_description:"V-type proton ATPase subunit E (AHRD V1 **** D3PGM0_9MAXI); contains Interpro domain(s) IPR002842 ATPase, V1/A1 complex, subunit E "
ref|XP_009779437.1|;PREDICTED: V-type proton ATPase subunit E-like [Nicotiana sylvestris]
NbS00041077g0001.1;protein AED:0.39 eAED:0.39 QI:0|0.8|0.66|0.83|1|1|6|0|245; (*GB) gi|225426050|ref|XP_002274995.1| (e_value=1e-122) PREDICTED: V-type proton ATPase subunit E [Vitis vinifera];; (*SWP) sp|Q9SWE7|VATE_CITLI (e_value=1e-122) V-type proton ATPase subunit E OS=Citrus limon GN=VATE PE=2 SV=1;; (*TAIR) AT4G11150.1 (e_value=7e-108) | Symbols: TUF, emb2448, TUFF, VHA-E1 | vacuolar ATP synthase subunit E1 | chr4:6800091-6801696 FORWARD LENGTH=230;; (*ITAG) Solyc12g056110.1.1 (e_value=3e-132) evidence_code:10F1H1E1IEG genomic_reference:SL2.40ch12 gene_region:47434718-47440701 transcript_region:SL2.40ch12:47434718..47440701+ functional_description:"V-type proton ATPase subunit E (AHRD V1 ***- D3PGM0_9MAXI); contains Interpro domain(s) IPR002842 ATPase, V1/A1 complex, subunit E ";
AT4G11150.1;| Symbols: TUF, emb2448, TUFF, VHA-E1 | vacuolar ATP synthase subunit E1 | chr4:6800091-6801696 FORWARD LENGTH=230
GO ID (Nr annotation)
GO:0033178 GO:0046961 GO:0015991
GO ID (Arabidopsis annotation)
GO:0033178 GO:0046961 GO:0015991
GO ID (Tomato annotation)
GO:0033178 GO:0046961 GO:0015991
GO ID (Niben annotation)
GO:0033178 GO:0046961 GO:0015991
Sequence
>NbTI01B04
ATGAACGACGCAGATGTATCGAAGCAGATCCAGCAGATGGTCAGATTCATCCGCCAGGAAGCCGAAGAAAAAGCTAATGAGATTTCCGTCTCCGCCGAAG
AAGAATTCAACATCGAGAAGTTGCAGCTAGTGGAACTGGAGAAGAAGAAGATCAGACAGGAATATGAGCGTAAGGAGAAACAAGTCGATGTTCGCAAGAA
AATTGAGTACTCCATGCAACTCAACGCCTCTCGAATCAAGGTTCTTCAAGCTCAGGATGACTTGGTCAGCTCCATGAAGGAGGCAGCATCAAAGGAGCTT
TTAAACGTCAGCCAGAACCACCATATTTATGAGAAGCTTCTGCAGGATCTTATTGTTCAGAGTTTGCTCAGACTTAAAGAGCCTTGCGTCCTACTACGTT
GTCGGGAAGATGATGTTTCCTTGGTGGAAGGGGTCTTGGATGCAGCAAAAGAGGAGTATGCAGAGAAGGCCCAGGTACACTCACCGGAGGTCATAATTGA
CCAAATCTACCTTCCATCAGCTCCATCACATCACAATGCTCATGGCTCTTCTTGCTATGGAGGAGTAGTTTTGGCTTCTCGAGATGGAAAAATTGTATGT
GAAAATACAGTTGATGCCAGATTGGAAAGTTGTGTTCCGTAAGAAACTACCAGAGGATTCGCAAGTGTCTATTCGGTCAGATTGCTGCCTAATGCG
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

4-1:1A1-1D12 [6-20-12]//1B4--2.JPG