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Details




NbTI01B05

Phenotype Description
Droopy, pale green, mottled leaves
Annotation
Solyc08g015620.2.1;genomic_reference:SL2.50ch08 gene_region:4765220-4773446 transcript_region:SL2.50ch08:4765220..4773446- functional_description:"Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes (AHRD V1 **-- B6U7J5_MAIZE)"
ref|XP_009797317.1|;PREDICTED: uncharacterized protein LOC104243765 isoform X1 [Nicotiana sylvestris]
NbS00003359g0006.1;protein AED:0.24 eAED:0.24 QI:0|1|1|1|0.6|0.66|6|406|275; (*GB) gi|255547516|ref|XP_002514815.1| (e_value=2e-128) conserved hypothetical protein [Ricinus communis];; (*TAIR) AT2G43945.1 (e_value=3e-123) | Symbols: | unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G59870.1); Has 292 Blast hits to 292 proteins in 84 species: Archae - 0; Bacteria - 122; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 124 (source: NCBI BLink). | chr2:18197995-18199988 REVERSE LENGTH=289;; (*ITAG) Solyc08g015620.2.1 (e_value=6e-165) genomic_reference:SL2.40ch08 gene_region:4765220-4773446 transcript_region:SL2.40ch08:4765220..4773446- functional_description:"Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes (AHRD V1 **-- B6U7J5_MAIZE)";
AT2G43945.1;| Symbols: | unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G59870.1); Has 292 Blast hits to 292 proteins in 84 species: Archae - 0; Bacteria - 122; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 124 (source: NCBI BLink). | chr2:18197995-18199988 REVERSE LENGTH=289
GO ID (Nr annotation)
GO ID (Arabidopsis annotation)
GO ID (Tomato annotation)
GO ID (Niben annotation)
GO:0005750 GO:0043209 GO:0070062 GO:0004222 GO:0008270 GO:0006122 GO:0009060 GO:0016485
Sequence
>NbTI01B05
TGTCCGTGAGGAACACAGAAAGAAAGGGGGAAAAACTCAAAAAGGGCTTTGGATTTCAAGAAAAGGGATTAGTAAAAAGTTGTGCTAGCAAAAGCCAATC
AAATAGTTAAAGCATTAGTTGTGGGCAAATCAAGAAAGCCTAAGAAAGAATGCTAACTTCTTCATTGAGGCTCCCTTTTTTGCCTCTTCCTCACTCCCAT
CTTGCATTTTCCCCTTTGAATTTCAATAAAGTTGTTCACTTTAACAACCCCAATTTTCCCTTTAGTTCTTCTATTTCCTCTTCCAAAAGATTTGCTTCAA
CAAACGCATCTTCTCGTGGACAGCAGACGTGGCCAGATGGAGATGCTGAACGCAACGATAATTTGAGGAATGAAGATGAATATGCAATTCAGATGTTTGG
TTCTGATGACGAACTCAGCTCTCAGATTCCAACTCAAGCTCAGTCTGTTGTTGAAGGATCAGGATCAGTTTTGGTAACAGAGTATAAGCCAGTTCCAGAT
GTGGACTATCTGCAGGAGTTGTTAGCAATTCAACAACAAGGTCCAAGAAATATTGGCTTTTTTGGGACACGAAATATGGGATTTATGCATCAAGAACTTA
TTGAGATTTTAAGCTATGCCTTGGTCATTACGAAAAATCACATCTTTACTTCTGGAGCAGCAGGCACCAATGCTGCTGTCATTAGAGGTGCTCTTCGTGC
TGAGAAACCAGAGCTGCTAACTGTGATATTGCCGCAAAGCTTGAAAAAACAACCTCCGGAAAGTCAGGAGCTACTGTCTAAAGTGAAACATGTGATAGAG
AAACCTCACAATGACCATCTCTCACTGCTAGAAGCTAGCA
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

1-1:1A1-1D12 [6-20-12]//1B5.JPG