Noble Research Institute, LLC   Noble Research Institute | VIGS Database  
      VIGS Phenomics and Functional Genomics Database  
Details




NbTI01B09

Phenotype Description
Severely stunted plants, no apical meristem, crinkled, mottled top leaves, thick, pale green leaves
Annotation
Solyc03g121370.2.1;genomic_reference:SL2.50ch03 gene_region:63530082-63534056 transcript_region:SL2.50ch03:63530082..63534056- functional_description:"30S ribosomal protein S8 (AHRD V1 ***- Q1HRQ6_AEDAE); contains Interpro domain(s) IPR000630 Ribosomal protein S8 "
gb|KGN54938.1|;hypothetical protein Csa_4G608140 [Cucumis sativus]
NbS00022778g0011.1;protein AED:0.22 eAED:0.22 QI:193|1|0.5|1|1|1|4|316|130; (*GB) gi|357125783|ref|XP_003564569.1| (e_value=3e-91) PREDICTED: 40S ribosomal protein S15a-1-like [Brachypodium distachyon];; (*SWP) sp|P42798|R15A1_ARATH (e_value=2e-92) 40S ribosomal protein S15a-1 OS=Arabidopsis thaliana GN=RPS15AA PE=2 SV=2;; (*TAIR) AT5G59850.1 (e_value=1e-93) | Symbols: | Ribosomal protein S8 family protein | chr5:24112499-24113084 REVERSE LENGTH=130;; (*ITAG) Solyc03g121370.2.1 (e_value=2e-44) genomic_reference:SL2.40ch03 gene_region:63530082-63534056 transcript_region:SL2.40ch03:63530082..63534056- functional_description:"30S ribosomal protein S8 (AHRD V1 ***- Q1HRQ6_AEDAE); contains Interpro domain(s) IPR000630 Ribosomal protein S8 ";
AT5G59850.1;| Symbols: | Ribosomal protein S8 family protein | chr5:24112499-24113084 REVERSE LENGTH=130
GO ID (Nr annotation)
GO:0005840 GO:0003735 GO:0006412
GO ID (Arabidopsis annotation)
GO:0005840 GO:0003735 GO:0006412
GO ID (Tomato annotation)
GO:0005840 GO:0003735 GO:0006412
GO ID (Niben annotation)
GO:0005840 GO:0003735 GO:0006412
Sequence
>NbTI01B09
GGCGAAGTAGATATTCGTGTTACCTTCCTCTCACCGAATCAAAGCGAAATAATGGTTAGGGTTAGTGTGCTGAATGATGCTCTCAAGAGCATGTACAATG
CTGAAAAGAGGGGAAAGCGTCAGGTCATGATTAGACCTTCCTCAAAAGTCATCATCAAGTTCCTCATTGTCATGCAGAAGCACGGTTACATTGGAGAATT
TGAGTACGTCGATGATCATAGGTCTGGAAAGATAGTTGTTGAGCTGAATGGAAGGTTAAACAAGTGTGGCGTCATTAGTCCTCGCTTTGATGTCGGAGTT
AAGGAGATTGAAGGATGGACTGCTAGATTACTCCCTTCTCGTCAGTTCGGCTACATTGTGCTGACAACTTCTGCTGGTATCATGGACCATGAGGAGGCCC
GCCGAAAGAATGTTGGTGGAAAAGTTCTCGGTTTCTTTTATTGAGTTTGTTATGTTGACAAGGATGAAAACTTTGTTTTTGCTGTTGGCTTTTTACGGTA
AATTAGCTACTTTTTGTTTGGGTTCATATCAATTTTAGTAGGGACTTTATTGAAATTTTGGAGACAATTATAAGCATTTGTTGACAAAAGATGTTGATGC
TTTTTGTGAACTTTGTGTGGTATGTCTAA
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:1A1-1D12 [6-20-12]//1B9b.JPG