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Details




NbTI01C05

Phenotype Description
Severely stunted plants, white green, mottled, crinkled, brittle leaves
Annotation
Solyc12g098890.1.1;evidence_code:10F1H1E1IEG genomic_reference:SL2.50ch12 gene_region:64598713-64601622 transcript_region:SL2.50ch12:64598713..64601622- functional_description:"50S ribosomal protein L18 (AHRD V1 ***- B6TYR5_MAIZE); contains Interpro domain(s) IPR004389 Ribosomal protein L18, bacterial "
ref|XP_009803342.1|;PREDICTED: 50S ribosomal protein L18, chloroplastic-like [Nicotiana sylvestris]
NbS00014933g0005.1;protein AED:0.37 eAED:0.37 QI:0|1|0.66|1|1|1|3|0|162; (*GB) gi|225432240|ref|XP_002270819.1| (e_value=3e-83) PREDICTED: 50S ribosomal protein L18, chloroplastic isoform 1 [Vitis vinifera];; (*SWP) sp|Q9SX68|RK18_ARATH (e_value=3e-77) 50S ribosomal protein L18, chloroplastic OS=Arabidopsis thaliana GN=RPL18 PE=1 SV=1;; (*TAIR) AT1G48350.1 (e_value=2e-78) | Symbols: | Ribosomal L18p/L5e family protein | chr1:17867269-17868215 FORWARD LENGTH=170;; (*ITAG) Solyc12g098890.1.1 (e_value=4e-106) evidence_code:10F1H1E1IEG genomic_reference:SL2.40ch12 gene_region:64598713-64601622 transcript_region:SL2.40ch12:64598713..64601622- functional_description:"50S ribosomal protein L18 (AHRD V1 ***- B6TYR5_MAIZE); contains Interpro domain(s) IPR004389 Ribosomal protein L18, bacterial ";
AT1G48350.1;| Symbols: | Ribosomal L18p/L5e family protein | chr1:17867269-17868215 FORWARD LENGTH=170
GO ID (Nr annotation)
GO:0005840 GO:0003735 GO:0006412
GO ID (Arabidopsis annotation)
GO:0005840 GO:0003735 GO:0006412
GO ID (Tomato annotation)
GO:0005840 GO:0003735 GO:0006412
GO ID (Niben annotation)
GO:0005840 GO:0003735 GO:0006412
Sequence
>NbTI01C05
AAAAGCCATGGCTTGTACTTCACTTTCACTTTCTTTTCTTCACAACGCTTGCGCCGATAACAAGCAGCTGACTCTTCCATTCCGTACCAAATTGGTGACG
TCAGCTCGGCCTCTTACAGTTGAAGCCAAGGCCACAACGAGACGGGAAGACAGAACTGCCCGACATGTTCGTATTCGGAAGAAGGTTGAAGGGACACCTG
AAAGACCAAGATTGTGTGTCTTCCGTTCCAACAAGCACATCTATGTTCAAGTAATTGATGACTCCAAGATGCACACACTGGCATCTGCTTCAACAATGCA
GAAACCAATCGCTGAGGAATTCGACTACTCCGCTGGCCCCACTACAGACGTGGCGAAGAAAGTTGGGGAGGTTATAGCTAAGGCCTGTCTAGAGAAAGGA
ATCACTAAAGTAGCCTTCGACCGAGGAGGTTATCCTTACCATGGACGGATTGAAGCTCTCGCTGATGCTGCAAGAGAACACGGCCTTCAGTTTTAGAAAG
TGCAGATTTCTTTGTTCTTCTTTTTTGTTGTGCAAATTACCTTTTCTGATCTTCCCATTTGTTCCCAGTTTGTAAAACTCATGTCTAAGAAGTAAGATGA
ACTGGAGTTTAAATGTATGATCTGCAATTCTTTTTGGTTTTATGGCTCCTTTAAATCAGAACTTATATTCCCTTTGTATTTCCAACTTTTTATGACATTG
CCTTTCAATTTTGAGA
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

3-1:1A1-1D12 [6-20-12]//1C5b--2.JPG