Noble Research Institute, LLC   Noble Research Institute | VIGS Database  
      VIGS Phenomics and Functional Genomics Database  
Details




NbTI01D07

Phenotype Description
Pale green leaves
Annotation
Solyc04g016260.2.1;genomic_reference:SL2.50ch04 gene_region:7057222-7066683 transcript_region:SL2.50ch04:7057222..7066683+ functional_description:"Calcium-transporting ATPase 1 (AHRD V1 **** Q7XBH9_CERRI); contains Interpro domain(s) IPR006408 ATPase, P-type, calcium-transporting, PMCA-type "
ref|XP_009800953.1|;PREDICTED: putative calcium-transporting ATPase 11, plasma membrane-type [Nicotiana sylvestris]
NbS00042193g0004.1;protein AED:0.22 eAED:0.22 QI:243|1|0.85|1|1|1|7|941|1070; (*GB) gi|291278198|gb|ADD91580.1| (e_value=0.0) calcium ATPase [Nicotiana benthamiana];; (*SWP) sp|Q9M2L4|ACA11_ARATH (e_value=0.0) Putative calcium-transporting ATPase 11, plasma membrane-type OS=Arabidopsis thaliana GN=ACA11 PE=2 SV=1;; (*TAIR) AT3G57330.1 (e_value=0.0) | Symbols: ACA11 | autoinhibited Ca2+-ATPase 11 | chr3:21211655-21216375 REVERSE LENGTH=1025;; (*ITAG) Solyc04g016260.2.1 (e_value=0.0) genomic_reference:SL2.40ch04 gene_region:7057222-7066683 transcript_region:SL2.40ch04:7057222..7066683+ functional_description:"Calcium-transporting ATPase 1 (AHRD V1 **** Q7XBH9_CERRI); contains Interpro domain(s) IPR006408 ATPase, P-type, calcium-transporting, PMCA-type ";
AT2G41560.1;| Symbols: ACA4 | autoinhibited Ca(2+)-ATPase, isoform 4 | chr2:17332256-17337179 REVERSE LENGTH=1030
GO ID (Nr annotation)
GO:0005887 GO:0043231 GO:0005388 GO:0005516 GO:0005524 GO:0046872 GO:0008152 GO:0070588
GO ID (Arabidopsis annotation)
GO:0005783 GO:0005829 GO:0005887 GO:0009986 GO:0030425 GO:0031090 GO:0044297 GO:0044446 GO:0008392 GO:0008395 GO:0016496 GO:0016712 GO:0046872 GO:0097159 GO:1901363 GO:0002118 GO:0002526 GO:0002687 GO:0003051 GO:0007166 GO:0007204 GO:0007217 GO:0007218 GO:0007616 GO:0008306 GO:0009408 GO:0010193 GO:0010634 GO:0010996 GO:0014910 GO:0019233 GO:0019369 GO:0032224 GO:0032230 GO:0032355 GO:0032570 GO:0035094 GO:0035106 GO:0035815 GO:0042713 GO:0042755 GO:0043117 GO:0043278 GO:0045471 GO:0045760 GO:0045777 GO:0045778 GO:0045907 GO:0046878 GO:0046887 GO:0048266 GO:0048660 GO:0050671 GO:0050679 GO:0051496 GO:0051602 GO:0060083 GO:0070474
GO ID (Tomato annotation)
GO:0005737 GO:0070062 GO:0004674 GO:0042803 GO:0007346 GO:0023014 GO:0031098 GO:0032147 GO:0033129 GO:0043065 GO:0046777 GO:0071356
GO ID (Niben annotation)
GO:0005615 GO:0005887 GO:0033017 GO:0000166 GO:0005102 GO:0043167 GO:0086039 GO:0006816 GO:0006874 GO:0006952 GO:0008152 GO:0010882 GO:0086036
Sequence
>NbTI01D07
TTGTCAATGTTGTGGCTCTGATGATCAATTTTATTTCTGCATGCGCCTCAGGATCTGCTCCTCTCACTGCTGTTCAGCTGCTTTGGGTCAACCTCATCAT
GGATACTTTAGGTGCATTGGCACTGGCCACTGAACCACCCCATGATGGATTAATGAGTCGGCCTCCTGTTGGAAGGGATGTGAGTTTCATTACAAAGACA
ATGTGGAGAAACATAATAGGACACAGTATCTACCAGCTGGCTATACTCTTAACCTTCAACTTTGCCGGAAAGCAGATTTTGAGACTTGAAGGTTCAGATG
CTACAAAAATCCAGAATACCTTCATTTTTAACACCTTTGTGTTTTGTCAGGTGTTCAACGAAATAAACAGTCGTGATATGGATAAGATAAACATTTTCCG
TGGCATTTTTAGCAGCTGGATTTTCTTAGGAGTCATGTTTGCTACAGTTGTTTTCCAAGTTATCATCATCGAGTTCTTAGGCACTTTTGCCAGCACTACA
CCACTTAGCTGGCAACTATGGCTGATCAGTGTCTTGATTGGTGCTGCAAGCTTGATTGTTGCTGTTATTTTGAAGTTGATCCCTGTCGAAAAGGAGACTT
CTAAGCACCATGACGGCTATGATCTGCTACCAAGTGGTCCAGAACTTGCCTAGGAGTGTGGTAATCAGATTCTTATGAGATATATGGTTTGCTTGGTCGA
ACTTCGGATGCTACTAGACCTGTTTGAGCCTTTGAGGTGAGTACGAAACAAAACAAATGGAAAGAAATACAAAATAAAGACTTAAAAACAGCT
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

1-1:1A1-1D12 [6-20-12]//1D7.JPG