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Details




NbTI01E06

Phenotype Description
Severely stunted, bushy plant, mottled, crinkled, yellow green leaves, spotted cell death
Annotation
Solyc01g079250.2.1;genomic_reference:SL2.50ch01 gene_region:70850920-70856753 transcript_region:SL2.50ch01:70850920..70856753- functional_description:"mRNA splicing factor ATP-dependent RNA helicase (AHRD V1 **** Q2UED4_ASPOR); contains Interpro domain(s) IPR014001 DEAD-like helicase, N-terminal "
ref|XP_009774703.1|;PREDICTED: probable pre-mRNA-splicing factor ATP-dependent RNA helicase [Nicotiana sylvestris]
NbS00018439g0015.1;protein AED:0.10 eAED:0.10 QI:0|1|1|1|0.83|0.85|7|443|722; (*GB) gi|356499785|ref|XP_003518717.1| (e_value=0.0) PREDICTED: probable pre-mRNA-splicing factor ATP-dependent RNA helicase-like [Glycine max];; (*SWP) sp|O22899|DHX15_ARATH (e_value=0.0) Probable pre-mRNA-splicing factor ATP-dependent RNA helicase OS=Arabidopsis thaliana GN=At2g47250 PE=2 SV=1;; (*TAIR) AT3G62310.1 (e_value=0.0) | Symbols: | RNA helicase family protein | chr3:23057516-23060561 REVERSE LENGTH=726;; (*ITAG) Solyc01g079250.2.1 (e_value=0.0) genomic_reference:SL2.40ch01 gene_region:70850920-70856753 transcript_region:SL2.40ch01:70850920..70856753- functional_description:"mRNA splicing factor ATP-dependent RNA helicase (AHRD V1 **** Q2UED4_ASPOR); contains Interpro domain(s) IPR014001 DEAD-like helicase, N-terminal ";
AT2G47250.1;| Symbols: | RNA helicase family protein | chr2:19399923-19402981 REVERSE LENGTH=729
GO ID (Nr annotation)
GO:0004386
GO ID (Arabidopsis annotation)
GO:0004386
GO ID (Tomato annotation)
GO:0004386
GO ID (Niben annotation)
GO:0004386
Sequence
>NbTI01E06
TTGCTAAACAAAAAGTTTATAACCCTAGGGTGCGTGTTGAATCTTTGTTGGTGTCTCCTATTTCGAAGGCCAGTGCCCACCAGAGATCAGGACGTGCTGG
AAGAACGCAGCCAGGGAAATGCTTTAGACTTTACACAGAGAAAAGTTTCCATAACGACCTTCAACCACAGACCTATCCAGAAATACTTCGGTCAAATTTG
GCTAATACAGTTCTAACTTTGAAGAAGTTAGGTATTGACGACCTGGTTCATTTTGATTTTATGGATCCTCCTGCTCCAGAGACGTTGATGAGAGCACTGG
AGGTTTTGAATTACTTGGGAGCATTGGATGATGAGGGGAACTTGACTAGGTTGGGTGAGATTATGAGTGAATTTCCTCTTGATCCTCAGATGGCAAAAAT
GCTTGTTGTCAGCTCAGAATTCAACTGCTCCAATGAAATCCTCTCAATATCCGCCATGCTATCAGCACCCAATTGCTTTGTCCGGCCTAGGGAGGCTCAG
AAAGCTGCAGATGAAGCAAAGAATCGGTTCGGTCACATAGATGGGGATCATTTGACGTTGCTGAATGTGTATCATGCCTACAAGCAGAACACGGAAGATC
CTCAATGGTGCTACGAGAATTTCATCAATCAGAGGGCTCTCAAATCAGCCGACAATGTGAGGCAACAGCTTTCTCGCATAATGGCCAGGTTCAACCTCAA
GTTATGCAGCACAGACTTCAACAGTCGCGACTACTACGTAAACATAAGAAAGGCTATGTTAGCTGGATATTTCATGCAGGTTGCTCACCTAGAGCGTACT
GGGCACTATCTGACTGTTAAAGACAATCAAGTGGTTCACTTGCATCCTTCAAATTGCCTGGATCATAAGCCA
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:1E1-1H12 [7-5-12]//1E6.JPG