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Details




NbTI01F02

Phenotype Description
Severely stunted plants, mottled, crinkled, small leaves, cell death, no apical meristem
Annotation
Solyc04g080590.2.1;genomic_reference:SL2.50ch04 gene_region:62295025-62299348 transcript_region:SL2.50ch04:62295025..62299348- functional_description:"Proteasome subunit alpha type (AHRD V1 ***- Q8H1Y2_NICBE); contains Interpro domain(s) IPR001353 Proteasome, subunit alpha/beta "
ref|XP_009771200.1|;PREDICTED: proteasome subunit alpha type-1-B-like [Nicotiana sylvestris]
NbS00005792g0020.1;protein AED:0.11 eAED:0.11 QI:148|1|0.5|1|0|1|2|0|274; (*GB) gi|22947842|gb|AAN07899.1| (e_value=1e-177) 20S proteasome alpha 6 subunit [Nicotiana benthamiana];; (*SWP) sp|P34066|PSA1A_ARATH (e_value=5e-154) Proteasome subunit alpha type-1-A OS=Arabidopsis thaliana GN=PAF1 PE=1 SV=3;; (*TAIR) AT5G42790.1 (e_value=3e-155) | Symbols: PAF1, ATPSM30, ARS5 | proteasome alpha subunit F1 | chr5:17159270-17160975 REVERSE LENGTH=278;; (*ITAG) Solyc04g080590.2.1 (e_value=8e-176) genomic_reference:SL2.40ch04 gene_region:62295025-62299348 transcript_region:SL2.40ch04:62295025..62299348- functional_description:"Proteasome subunit alpha type (AHRD V1 ***- Q8H1Y2_NICBE); contains Interpro domain(s) IPR001353 Proteasome, subunit alpha/beta ";
AT5G42790.1;| Symbols: PAF1, ATPSM30, ARS5 | proteasome alpha subunit F1 | chr5:17159270-17160975 REVERSE LENGTH=278
GO ID (Nr annotation)
GO:0019773 GO:0004298 GO:0006511
GO ID (Arabidopsis annotation)
GO:0019773 GO:0004298 GO:0006511
GO ID (Tomato annotation)
GO:0019773 GO:0004298 GO:0006511
GO ID (Niben annotation)
GO:0019773 GO:0004298 GO:0006511
Sequence
>NbTI01F02
ACTCCAAAAAACGACATCGTTTTATAATTCCTAACATTTGACAAACAGAATGTTCAGAAACCAGTACGATACCGACGTGACGACATGGTCACCGGCGGGG
AGGCTATTTCAGGTAGAGTACGCAATGGAGGCAGTGAAGCAAGGTTCAGCGGCACTAGGTTTGAGATCGAAGACGCACGTGATTCTCGCGTGCGTTAATA
AGGCGAATTCCGAGTTATCCTCTCACCAGAAGAAGATCTTCAAAGTTGATGACCACATCGGAGTTGCAATCGCCGGACTTACTGCCGATAGCCGTGTACT
TTCTCGTTATATGCGTAATGAGTGCATTAATTATAGTTACGCTTATGAGTCGCCGTTTCCTGTAGGCCGCCTTGTTGTTCAGCTCGCTGATAAAGCTCAG
GTCTGCACTCAACGATCCTGGAAACGGCCTTATGGTGTTGGCCTGCTTGTTGGTGGGCTGGATGAGTCTGGTGCTCACCTTTATTACAATTGCCCTAGTG
GTAACTACTTCGAGTATCAAGCTTTTGCCATTGGATCTCGATCACAAGCTGCCAAAACCTACTTGGAACGTAGGTTTGAGACTTTCATGGATTCTTCACG
GGAAAGCCTGCTTAAGGACGCACTCTTTGCACTGAGAGAAACTTTGCAGGGAGAAAAACTAACAAGCACCAATTGCACGGTTGCAGTGGTAGGAGTAGGA
GAGGCTTTTCATATGTTGGACAAGGAAACTATACAAGCATTGATCAATGAATTTGAGATAGCTGGTGAAGAAGCTCCTGCTGCAGCCCCAGATGAAGCTG
GTAGTGATGAACCGGCAGCAGCACCGCCTGCTGATCAGGGAGCTGCCCCGATGGATATTTGATGATGTTATGTCCTCCCTTTAGGCCTCACTTTTTT
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:1E1-1H12 [7-5-12]//1F2.JPG