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Details




NbTI01F05

Phenotype Description
Stunted, bushy plants, more branches (loss of apical dominance), mottled, chlorotic top leaves
Annotation
Solyc03g096840.2.1;genomic_reference:SL2.50ch03 gene_region:52656957-52659784 transcript_region:SL2.50ch03:52656957..52659784- functional_description:"Seed specific protein Bn15D1B (AHRD V1 ***- B6TIB8_MAIZE)"
ref|XP_009757404.1|;PREDICTED: uncharacterized protein LOC104210248 isoform X2 [Nicotiana sylvestris]gb|ACK38177.1| hydrogen peroxide-induced 1 [Nicotiana tabacum]
NbS00023612g0003.1;protein AED:0.00 eAED:0.00 QI:599|1|1|1|1|1|2|884|128; (*GB) gi|217323620|gb|ACK38177.1| (e_value=9e-78) hydrogen peroxide-induced 1 [Nicotiana tabacum];; (*TAIR) AT3G03150.1 (e_value=3e-25) | Symbols: | unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G17165.1); Has 39 Blast hits to 39 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 39; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). | chr3:727733-729357 REVERSE LENGTH=121;; (*ITAG) Solyc03g096840.2.1 (e_value=2e-70) genomic_reference:SL2.40ch03 gene_region:52656957-52659784 transcript_region:SL2.40ch03:52656957..52659784- functional_description:"Seed specific protein Bn15D1B (AHRD V1 ***- B6TIB8_MAIZE)";
AT3G03150.1;| Symbols: | unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G17165.1); Has 39 Blast hits to 39 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 39; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). | chr3:727733-729357 REVERSE LENGTH=121
GO ID (Nr annotation)
GO ID (Arabidopsis annotation)
GO ID (Tomato annotation)
GO ID (Niben annotation)
Sequence
>NbTI01F05
TTCAGATTAGTGTGTAAGAGAGAAATTCTATACGAAACTCCATTTCTTCTTATTCTTTAGTGTATATTGTATGGCCGCCAATTTGCAGAGCCGGGGACTC
GTGAGCTTAGGGAAACGAGTTGTGAACCAGATCAGCTACGCTAGTGCTCGAACTTCTGCTAACTCACCTTCACTCTCTGACAGGAGGGGAGTGCAGACAT
CGGTGTATGACAAGAACCCGGAGGATCATGTGCGGGATTCGGTGGTGCCGGATGAAGTGATAGAGCTACAATCAGAAAAATACTGGGGTCCTCACCCACA
AACTGGGGTATTTGGGCCTGCAGCCACTAATAGTGCTGGTGGGGAACGCGGTTTCCACCACTCACCTGCCACTGCTGCTGCTGCTGCTGAATCCATTTTG
GAGCAGAAGACCTTCTTCCGCCCTCTCGAGGACTTGGACAAACCCCAGCACGCCTAAGTTTCACTTTCCTTAAATAGCTCCAGTGTGTCTATCCTCACTT
GCAACTTAAGTAATTGCTCTATAACTATATATAGTGTGTAGTAGTGACTTTTTAAATAAGTTTCTGTTTGCTTTGGCAAATGCGAGTCTGTATATCAAGT
TTAAAGTGCAGTATAGACTATTAGAAAGCTGCACCGCTGTTGAAGGTTCCGTTCATATGGTACTGCAATATGGGTGTCTGTTGTTACAGTTACGTAACTG
CTGAACTATGCTATAATATCCTTATGAACTTTTGGCTTTCG
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

1-1:1E1-1H12 [7-5-12]//1F5.JPG