Noble Research Institute, LLC   Noble Research Institute | VIGS Database  
      VIGS Phenomics and Functional Genomics Database  
Details




NbTI02D02

Phenotype Description
Cell death, nearly dead plants
Annotation
Solyc10g081510.1.1;evidence_code:10F1H1E1IEG genomic_reference:SL2.50ch10 gene_region:61890862-61894137 transcript_region:SL2.50ch10:61890862..61894137+ functional_description:"5- methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (AHRD V1 ***- B6UF55_MAIZE); contains Interpro domain(s) IPR006276 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase "
ref|XP_009804811.1|;PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like [Nicotiana sylvestris]ref|XP_009804812.1| PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like [Nicotiana sylvestris]ref|XP_009804813.1| PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like [Nicotiana sylvestris]
NbS00012577g0009.1;protein AED:0.13 eAED:0.13 QI:321|1|1|1|0.9|0.81|11|196|765; (*SWP) sp|Q42699|METE_CATRO (e_value=0.0) 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus GN=METE PE=2 SV=1;; (*TAIR) AT5G17920.2 (e_value=0.0) | Symbols: ATCIMS | Cobalamin-independent synthase family protein | chr5:5935771-5939195 FORWARD LENGTH=765;; (*ITAG) Solyc10g081510.1.1 (e_value=0.0) evidence_code:10F1H1E1IEG genomic_reference:SL2.40ch10 gene_region:61890862-61894137 transcript_region:SL2.40ch10:61890862..61894137+ functional_description:"5- methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (AHRD V1 ***- B6UF55_MAIZE); contains Interpro domain(s) IPR006276 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase ";
AT3G03780.3;| Symbols: ATMS2, MS2 | methionine synthase 2 | chr3:957602-960740 FORWARD LENGTH=765
GO ID (Nr annotation)
GO:0003871 GO:0008270 GO:0009086
GO ID (Arabidopsis annotation)
GO:0003871 GO:0008270 GO:0009086
GO ID (Tomato annotation)
GO:0003871 GO:0008270 GO:0009086
GO ID (Niben annotation)
GO:0003871 GO:0008270 GO:0009086
Sequence
>NbTI02D02
TTGGAGAAGGCAGGCATCACTGTCATCCAAATCGATGAAGCTGCATTGAGAGAGGGGTTGCCTCTTAGGAAGGCTGAGCATGCTTTCTACTTGAACTGGG
CCGTTCACTCCTTCAGAATCACCAATGTCAATATCCAGGACACTACCCAGATCCACACTCACATGTGCTACTCCAACTTCAATGACATTATCCACTCCAT
CATCGACATGGATGCTGATGTGATCACAATTGAGAACTCACGTTCCGATGAGAAGCTCCTCTCAGTTTTCAGGGAGGGAGTGAAGTATGGTGCTGGAATT
GGCCCTGGTGTCTATGACATCCACTCTCCAAGAATACCATCAACCGAAGAAATTGCTGATAGAGTGAACAAGATGCTTGCTGTTCTTGACACCAACATCT
TGTGGGTCAACCCTGACTGTGGTCTCAAGACCCGCAAGTACACCGAGGTGAAGCCAGCACTCCAAGCCATGGTTTCTGCAGCCAAGGCCATCCGCACCCA
GCTTGCCAGCGCCAAGTGAGCCAGTTGGAACTTGGAAAGGAAATTTTCTATTGATTTTTAATAATTGTGCTTGCTATGATATTATTCTGTAGGTTAGTTG
TACTGCCCCCCGGGCATTC
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:2A1-2D12[[7-10-12]//2D2..JPG