Noble Research Institute, LLC   Noble Research Institute | VIGS Database  
      VIGS Phenomics and Functional Genomics Database  
Details




NbTI02F05

Phenotype Description
Moderately stunted plants, spotted cell death in top leaves, pale green top leaves
Annotation
Solyc06g054260.1.1;evidence_code:10F1H1E1IEG genomic_reference:SL2.50ch06 gene_region:33565231-33565857 transcript_region:SL2.50ch06:33565231..33565857- functional_description:"Photosystem I reaction center subunit II (AHRD V1 ***- B4FAW3_MAIZE); contains Interpro domain(s) IPR003685 Photosystem I protein PsaD "
ref|XP_009621208.1|;PREDICTED: photosystem I reaction center subunit II, chloroplastic [Nicotiana tomentosiformis]
NbS00018749g0002.1;protein AED:0.25 eAED:0.25 QI:0|-1|0|1|-1|1|1|0|207; (*GB) gi|131167|sp|P29302.1|PSAD_NICSY (e_value=6e-131) RecName: Full=Photosystem I reaction center subunit II, chloroplastic; AltName: Full=Photosystem I 20 kDa subunit; Short=PSI-D; Flags: Precursor;; (*SWP) sp|P29302|PSAD_NICSY (e_value=2e-132) Photosystem I reaction center subunit II, chloroplastic OS=Nicotiana sylvestris GN=psaD PE=2 SV=1;; (*TAIR) AT1G03130.1 (e_value=4e-102) | Symbols: PSAD-2 | photosystem I subunit D-2 | chr1:753528-754142 REVERSE LENGTH=204;; (*ITAG) Solyc06g054260.1.1 (e_value=1e-121) evidence_code:10F1H1E1IEG genomic_reference:SL2.40ch06 gene_region:33565231-33565857 transcript_region:SL2.40ch06:33565231..33565857- functional_description:"Photosystem I reaction center subunit II (AHRD V1 ***- B4FAW3_MAIZE); contains Interpro domain(s) IPR003685 Photosystem I protein PsaD ";
AT1G03130.1;| Symbols: PSAD-2 | photosystem I subunit D-2 | chr1:753528-754142 REVERSE LENGTH=204
GO ID (Nr annotation)
GO:0009538 GO:0015979
GO ID (Arabidopsis annotation)
GO:0009538 GO:0015979
GO ID (Tomato annotation)
GO:0009538 GO:0015979
GO ID (Niben annotation)
GO:0009538 GO:0015979
Sequence
>NbTI02F05
GGATCACTTTTGTAAGCCAATAATTCTACAATGGCCATGGCAACTCAAGCTTCTCTCTTCACTCCAGCTCTCTCTGCCTCAAAATCTTCAGCCCCATGGA
AACAATCCCTTGCTTCCTTCTCTCCTAAGCAACTCAAATCCACTGCTTCCACTCCCCGTCCCATTAGAGCCATGGCCGAAGAAGCCGCCGCCGCCGCCAC
AACAAAAGCAGAGGCTCCAGTGGGCTTTACCCCACCACAATTGGACCCAAACACACCTTCCCCAATCTTCGGTGGCAGCACCGGCGGGCTTCTCCGCAAG
GCCCAAGTTGAGGAGTTTTACGTAATTACTTGGGAATCACCTAAAGAACAGATCTTTGAGATGCCAACTGGTGGTGCAGCTATTATGAGGGAAGGTGCTA
ATTTGCTGAAATTGGCGAGGAAAGAGCAGTGTTTAGCACTTGGTACTAGGCTTAGGTCAAAGTACAAGATTAACTACAGGTTTTACAGGGTGTTTCCTAA
TGGTGAGGTTCAATACCTGCACCCTAAGGATGGTGTGTACCCAGAAAAGGTGAACGCTGGCCGTGAAGGAGTTGGACAGAACTTCAGATCCATTGGGTAG
GAACAAGAGCCCAATTGAGGTCAAGTTCACTGGCAAACAAGTGTATGATTTGTAAGCTGA
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:2E1-2H12[7-9-12]//2F5..JPG